Proinflammatory cytokines in peritoneal fluid of women with peritoneal adhesions




peritoneal fluid, cytokines, adhesion process, peritoneal pelvic adhesions


Purpose - to investigate the level of pro-inflammatory cytokines in the peritoneal fluid in women of reproductive age with peritoneal adhesions.

Materials and methods. 58 patients (the main group) verified during laparoscopy were included in the study: of them, 45 patients with stages I-II of the adhesion process, 13 - with stages III-IV of the spread of the process. The control group (CG) consisted of 30 women of reproductive age with intact reproductive function without adhesions in the pelvis, who underwent laparoscopic sterilization. The content of interleukin (IL) 1, IL-2, IL-6 and tumor necrosis factor alpha (TNF-α) in the peritoneal fluid was analyzed.

Results. The study of the content of proinflammatory cytokines in peritoneal fluid showed that their levels were significantly higher (p<0.01) in women with stage I-II and stage III-IV adhesions: IL-1 - 192.5±34.8 pg/ml and 268.4±48.3 pg/ml, respectively; IL-2 - 145.9±21.3 pg/ml and 234.8±42.5 pg/ml, respectively; IL-6 - 118.7±8.4 pg/ml and 698.4±22.6 pg/ml, respectively; TNF-α - 10.2±4.8 pg/ml and 29.6±2.8 pg/ml, respectively. These levels were the lowest in CG women: IL-1 - 18.2±4.2 pg/ml; IL-2 - 22.4±4.3 pg/ml; IL-6 - 26.4±4.7 pg/ml; and TNF-α was not detected. The level of IL-6 in peritoneal fluid of patients with stages III-IV of the adhesive process (698.4±22.6 pg/ml) was significantly higher (p<0.01) than that in women with stages I-II of adhesion prevalence (118.7±8.4 pg/ml) and in women of the CG (26.4±4.7 pg/ml).

Conclusions. With an increase in the prevalence of the adhesive process, the content of cytokines in the peritoneal fluid increases significantly, and the peritoneal fluid plays a significant role in its formation.

The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies.

No conflict of interests was declared by the author.


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