State of local cervical immunity in women with infertility




infertility, interleukins, early cervical lesions, lactoferrin, secretory leukocyte proteinase inhibitor


Purpose - to study certain indicators of local immunity in women with infertility of various origins and early cervical lesions

Materials and methods. The state of local cervical immunity was examined in 496 women, who were divided into groups depending on the cause of infertility, and each group was divided into subgroups A and B depending on the presence of cervical epithelial lesions. 128 women in the Group I had an endocrine cause, 122 patients in the Group II had a tubal-peritoneal factor of infertility, and 121 patients in the Group III had a male factor of fertility disorder. 125 healthy women formed the control group (CG). Subgroup A included women with NILM liquid cytology results: IA - 100 women, IIA - 70, IIIA - 68, GCA - 88. Subgroups B included women with ASC-US (atypical squamous cells of undetermined significance), LSIL (low-grade squamous intraepithelial lesion) and HSIL (high-grade squamous intraepithelial lesion): IB - 28 patients, IIB - 52, IIIB - 53, CGB - 37. In all patients, the content of interleukin-8 and -6 (IL-8, IL-6), lactoferrin and secretory leukocyte proteinase inhibitor in the material obtained for liquid cytology was determined by polymerase chain reaction.

Results. In all patients with infertility, an increased concentration of secretory leukocyte proteinase inhibitor (from 314.1 to 398.7 pg/ml) and a decreased lactoferrin content (from 13.6 to 28.6 pg/ml) were found compared to the group of healthy women (114.8 and 64.8 pg/ml, respectively). In women with tubal and peritoneal infertility and male infertility, higher concentrations of IL-8 were found (659.9 to 878.8 pg/ml) than in the group of endocrine infertility (302.9 pg/ml) and in the group of healthy women (282.4 pg/ml).

Conclusions. IL-8 content in cervical mucus is probably higher in women with tubal-peritoneal infertility (838 pg/ml) and male infertility (659.9 pg/ml) than in healthy women (289.4 pg/ml) and in women with endocrine infertility (302.3 pg/ml). The content of secretory inhibitor of leukocyte proteinase in cervical mucus was increased in all patients with infertility, regardless of its cause (from 314.1 pg/ml to 398.9 pg/ml), in the group of healthy women - 144.8 pg/ml. 3. The content of lactoferrin in the mucus of the cervical canal was reduced in all patients with impaired fertility (from 13.6 pg/ml to 28.6 pg/ml), in the group of healthy women - 64.8 pg/ml.

The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies.

No conflict of interests was declared by the author.


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